The Macromolecular Crystallography Research Centre

Dr. Anat Shahar
Head

X-ray crystallography is the gold standard for determining biological structures at high resolution. Protein crystals are subjected to X-ray radiation, and the pattern of diffraction obtained is used to solve the structure of the molecule. X-ray crystallography thus provides vital information about structure-function relationships and protein-protein and protein-ligand interactions. The Macromolecular Crystallography Research Centre (MCRC) provides expert services needed for determining the three-dimensional structure of a protein. These services begin with protein crystallization and can continue through to crystal measurement and three-dimensional structural analysis. The MCRC also offers advanced robotics instrumentation to facilitate mass screening and imaging.

Sananes A., Cohen I., Shahar A., Hockla A., De Vita E., Miller A.K., Radisky E.S. and Papo N.(2018)
A potent, proteolysis-resistant inhibitor of kallikrein-related peptidase 6 (KLK6) for cancer therapy, developed by combinatorial engineering.
JBC.
Zur Y., Rosenfeld L., Bakhman A., Ilic S., Hayun H., Shahar A., Akabayov B., Kosloff M., Levaot N. and Papo N. (2017)
Engineering a monomeric variant of macrophage colony-stimulating factor (M-CSF) that antagonizes the c-FMS receptor.
Biochemistry Journal 474(15):2601-2617.
Vainer R., Cohen S., Shahar A., Zarivach R. and Arbely E. (2016)
Structural Basis for p53 Lys120-Acetylation-Dependent DNA-Binding Mode.
JMB 428(15):3013-3025.
Kanteev M., Bregman-Cohen A., Deri B., Shahar A., Adir N. and Fishman A. (2015)
A crystal structure of 2-hydroxybiphenyl 3-monooxygenase with bound substrate provides insights into the enzymatic mechanism.
BiochimBiophysActa. 1854(12):1906-1913.
Dror A., Kanteev M., Kagan I., Gihaz S., Shahar A. and Fishman A. (2015)
Structural insights into methanol-stable variants of lipase T6 from Geobacillusstearothermophilus.
Appl. Microbiol. Biotechnol. 99(22):9449-9461.