Cryo-Electron Microscopy
Dr. Ran ZalkHead
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The Electron Microscopy unit provides the expertise and resources for solving macromolecular structures. For structural determination, a high-end transmission electron microscope (FEI Tecnai F30 Polara), a direct detection camera (Gatan K2 Summit with Quantum GIF energy filter), and the computational support necessary for single particle analysis are available. Higher order structure determination is possible through tomographic reconstruction using the same setup. The unit also offers grid sample preparation expertise and equipment (Leica EM GP).
The equipment and services of the Electron Microscopy Unit are available for use by NIBN members and BGU faculty, as well as researchers at other universities and in industry.
- Email: ranz@post.bgu.ac.il
- Visit Website
Main Services
Expertise and resources for solving macromolecular structures
Grid sample preparation expertise and equipment (Leica EM GP)
Single particle analysis for structural determination (Gatan K2 Summit with Quantum GIF energy filter)
Publications
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Hartel A.J.W., Ong P., Schroeder I., Giese M.H., Shekar S., Clarke O.B., Zalk R., Marks A.R., Hendrickson W.A. and Shepard K.L. (2018)
Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes. Proc. Natl.
Proc. Natl. Acad. Sci. USA 115(8):E1789-E1798. -
Hartel A.J.W., Ong P., Schroeder I., Giese M.H., Shekar S., Clarke O.B., Zalk R., Marks A.R., Hendrickson W.A. and Shepard K.L. (2018)
Single-channel recordings of RyR1 at microsecond resolution in CMOS-suspended membranes.
Proc. Natl. Acad. Sci. USA115(8):E1789-E1798. -
Lacampagne A., Liu X., Reiken S., Bussiere R., Meli A.C., Lauritzen I., Teich A.F., Zalk R., Saint N., Arancio O., Bauer C., Duprat F., Briggs C.A., Chakroborty S., Stutzmann G.E., Shelanski M.L., Checler F., Chami M. and Marks A.R. (2017)
Post-translational remodeling of ryanodine receptor induces calcium leak leading to Alzheimer's disease-like pathologies and cognitive deficits.
Acta. Neuropathol. 134(5):749-767. -
Zalk R. and Marks A.R. (2017)
Ca2+ Release Channels Join the 'Resolution Revolution'.
Trends Biochem. Sci. 42(7):543-555. -
Bussiere R., Lacampagne A., Reiken S., Liu X., Scheuerman V., Zalk R., Martin C., Checler F., Marks A.R. andChami M. (2017)
Amyloid β production is regulated by β2-adrenergic signaling-mediated post-translational modifications of the ryanodine receptor.
J. Biol Chem. 292(24):10153-10168. -
des Georges A., Clarke O.B., Zalk R., Yuan Q., Condon K.J., Grassucci R.A., Hendrickson W.A., Marks A.R. and Frank J. (2016)
Structural Basis for Gating and Activation of RyR1.
Cell 167(1):145-157.e17.